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Literature summary extracted from

  • Di Bernardino, M.; Dimroth, P.
    Synthesis of the oxaloacetate decarboxylase Na+ pump and its individual subunits in Escherichia coli and analysis of their function (1995), Eur. J. Biochem., 231, 790-801.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
7.2.4.2 all subunits, expressed in Escherichia coli, alpha-subunit strongly overexpressed from pT7-7 plasmid Klebsiella pneumoniae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
7.2.4.2 Na+
-
 Klebsiella pneumoniae
7.2.4.2 Zn2+ gamma-subunit, involved in carboxytransfer, 0.96 mol/mol enzyme Klebsiella pneumoniae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
7.2.4.2 oxaloacetate Klebsiella pneumoniae Na+ transport coupled to oxaloacetate decarboxylation ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
7.2.4.2 Klebsiella pneumoniae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
7.2.4.2
-
 Klebsiella pneumoniae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
7.2.4.2 additional information
-
 activity of reconstituted enzyme and isolated subunits Klebsiella pneumoniae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7.2.4.2 additional information transmembrane transport of Na+ Klebsiella pneumoniae ?
-
 ?
7.2.4.2 Oxaloacetate
-
 Klebsiella pneumoniae Pyruvate + CO2
-
 ?
7.2.4.2 oxaloacetate Na+ transport coupled to oxaloacetate decarboxylation Klebsiella pneumoniae ?
-
 ?

Subunits

EC Number Subunits Comment Organism
7.2.4.2 trimer alpha-subunit also necessary for Na+ transport, spatial arrangement and subunit interactions Klebsiella pneumoniae